Different Methods for DNA Purification
DNA purification is one of the most common and important procedures in molecular biology. Purification of DNA click this link now aims at making it possible to separate the desired genetic material (chromosomal material) from harmful substances like proteins as well as RNA and cell membrane. This is an essential step in virtually all molecular applications and must be performed correctly to get high-quality, usable DNA.
There are a variety of methods for DNA purification. The choice is based on a variety of variables including the starting materials and downstream applications, as well as cost, and time constraints. Common genomic and plasmid DNA purification methods include chemical treatment, enzymatic digestion, or mechanical disruption of tissue or cell samples followed by salting-out the proteins and the precipitation of DNA using alcohol.
Ethanol precipitation is an inexpensive, quick and simple method of desalting and concentrating DNA. DNA molecules accumulate in the presence monovalent cations like sodium, and then they are removed out of solution using high levels of ethanol. This technique allows the removal of organic compounds, and other impurities in a sample. It is frequently used in conjunction with other purification techniques.
Anion exchange chromatography is yet another well-known method for DNA purification. DNA in a solution gets bound to positively charged resins due to the interaction between the negatively charged DNA phosphate backbone and the positively charged surface molecules of the resin. During the binding steps the contaminants are removed using a stringent washing process. The DNA that is purified is eluted under low salt conditions.